Application

Frozen Sections (see application references)r>Immunofluorescence (see comments)r>Paraffin Sections (see comments, heat pre-treatment required)r>Radial Immunodiffusion (use undiluted)

General description

Recognizes complement component complex Cb5-C9.

Protein A purified rabbit polyclonal antibody. Recognizes the C5b-C9 complement component complex.

This Anti-Complement 5b-9 Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence, Paraffin Sections, Radial Immunodiffusion for the detection of Complement 5b-9.

Immunogen

purified human SC5b-9 complex

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

By immunodiffusion the antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma. Also reported to work for immunofluorescence. This antibody has been shown to work for formalin-fixed paraffin sections; use of citrate buffer and a pressure cooker for 1 min is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.

Schafer, H., et al. 1986. J. Immunol.137, 1945.

Packaging

1 mg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.2.

Reconstitution

Following intial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)